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Advanced Life Cell Imaging

Vak 2011-2012

Actual course information on this course can be found here

This lecture series focuses on modern life cell imaging and it’s role in life science research. The cell can be considered the elementary quantum in modern life sciences and the use of life cell imaging techniques to study sub-cellular and molecular structures in living cells is discussed. In addition the high resolution imaging of single cells in tissue and complete organisms is discussed.
Each session is accompanied by home exercises that involve some practical work ranging from simple experiments to writing a simple program or using free software to calculate and visualize concepts discussed.

  • Basics of quantitative optical image formation using photons – Basics of fluorescence microscopy – Resolution and localisation of bio molecules, tags and their properties – Thick samples, non-fluorescent imaging methods – Breaking the Abbe limit – Pulses, probes and dynamic tagging – Unravelling probe dynamics and biology – Optimizing resolution in the space and time domain – Overlaying imaging techniques – Future bio probes, PALM, STORM, STED and X-ray microscopy

Prerequisites info: BSc in Life Sciences or Chemistry

Additional info:
Enrollment via Blackboard
One month before the course starts a kick off project will be announced.
The courses includes some aspects of quantitative image analysis and computation (mandatory excercises) and the examination will be the writing of an essay.
The use of a laptop during the course is recommended.