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Advanced Life Cell Imaging (ALCI)

Vak 2012-2013

"Actual course information":https://masters.lic.leidenuniv.nl/courses
"Up to date schedule information":https://masters.lic.leidenuniv.nl/download-schedule

This lecture series focuses on modern life cell imaging and it’s role in life science research. The cell can be considered the elementary quantum in modern life sciences and the use of life cell imaging techniques to study sub-cellular and molecular structures in living cells is discussed. In addition the high resolution imaging of single cells in tissue and complete organisms is discussed.
Each session is accompanied by home exercises that involve some practical work ranging from simple experiments to writing a simple program or using free software to calculate and visualize concepts discussed. We will use the program R to setup some simple programming and use the program ImageJ to visualize experiments.

Topics covered:

Basics of quantitative optical image formation using photons
Basics of fluorescence microscopy
Resolution and localisation of bio molecules, tags and their properties
Thick samples, non-fluorescent imaging methods
Breaking the Abbe limit
Pulses, probes and dynamic tagging
Unravelling probe dynamics and biology
Optimizing resolution in the space and time domain
Overlaying imaging techniques
Future bio probes, PALM, STORM, STED and X-ray microscopy

Literature:

The following two books best illustrate the scope of the lectures. Digital Image Processing, Burger Burge, Springer (recommended, not required)
Live Cell Imaging, Goldman & Spector, CSHLP (2nd edition, not required)
Assessment method:
Essay and homework
Prerequisites info:
BSc in Life Science or Chemistry
Additional info:
Enroll via Blackboard (non-automatic enrollment)