"Actual course information":https://masters.lic.leidenuniv.nl/courses
"Up to date schedule information":https://masters.lic.leidenuniv.nl/download-schedule
This lecture series focuses on modern life cell imaging and it’s role in life science research. The cell can be considered the elementary quantum in modern life sciences and the use of life cell imaging techniques to study sub-cellular and molecular structures in living cells is discussed. In addition the high resolution imaging of single cells in tissue and complete organisms is discussed.
Each session is accompanied by home exercises that involve some practical work ranging from simple experiments to writing a simple program or using free software to calculate and visualize concepts discussed. We will use the program R to setup some simple programming and use the program ImageJ to visualize experiments.
Basics of quantitative optical image formation using photons Basics of fluorescence microscopy Resolution and localisation of bio molecules, tags and their properties Thick samples, non-fluorescent imaging methods Breaking the Abbe limit Pulses, probes and dynamic tagging Unravelling probe dynamics and biology Optimizing resolution in the space and time domain Overlaying imaging techniques Future bio probes, PALM, STORM, STED and X-ray microscopy
The following two books best illustrate the scope of the lectures. Digital Image Processing, Burger Burge, Springer (recommended, not required)
Live Cell Imaging, Goldman & Spector, CSHLP (2nd edition, not required)
Essay and homework
BSc in Life Science or Chemistry
Enroll via Blackboard (non-automatic enrollment)