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Capillary Electrophoresis-Mass Spectrometry for Metabolomics

Vak 2019-2020

This information is without prejudice. Alterations can be made for next year

Description

Capillary electrophoresis-mass spectrometry (CE-MS) has become a very useful analytical technique for the profiling of highly polar and charged metabolites in biological samples. In this course, the unique features of CE-MS for metabolomics studies are highlighted and a comprehensive overview of recent technological developments will be given. Special emphasis will be given to advanced interfacing techniques for coupling CE to MS and on their implications for metabolomics. The applicability of CE-MS for metabolomics will be demonstrated using samples ranging from plasma and urine to cells and tissues. In particular, the utility of CE-MS for metabolic profiling of volume-restricted samples will be considered. An important aspect of the course will be the writing of a research proposal focused on the development of a CE-MS approach for addressing a relevant biological/clinical question using metabolomics. In this context, the candidate has full freedom to come up with an idea as long as the technological part is focused on CE or CE-MS method development.

Coordinator
Dr. Rawi Ramautar (r.ramautar@lacdr.leidenuniv.nl), group leader Biomedical Microscale Analytics (https://www.universiteitleiden.nl/en/science/drug-research/systems-pharmacology/biomedical-microscale-analytics)

Admission requirements
Master students BPS. The course is also open to third-year BFW students and to other MSc students with a basic background in analytical chemistry or separation sciences.

Mode of instruction
Lectures, self-study and student presentations.

Reading list
The following book is recommended for this course: Capillary Electrophoresis-Mass Spectrometry for Metabolomics by Rawi Ramautar, ISBN: 9781788012737, publisher: The Royal Society of Chemistry (http://pubs.rsc.org/bookshop/search?searchtext=ramautar)

Course objectives
At the end of the course, the candidate is able to:
• explain the working principles of capillary electrophoresis-mass spectrometry (CE-MS)
• explain the concept of metabolomics and how to set-up a CE-MS-based analytical workflow to address a biological/clinical question with metabolomics
• give an overview of the main technological developments in CE-MS for metabolomics studies
• discuss the strengths and limitations of CE-MS for metabolomics studies
• to write a research proposal on the development of a CE-MS approach for addressing a biological/clinical question using the Veni grant criteria of the Netherlands Organization for Scientific Research

Assessment method
For this course, you have to write a research proposal and to present the idea of the research proposal, also known as interview, towards your peers and the coordinator. The research proposal is 70% of the final grade and the interview is 30% of the final grade. For each part, the minimum grade should be a 5.5 in order to pass the course.

Timetable
November 2019. The specific schedule for this course will be published on Blackboard.

Registration
Application via uSis. Registration closes 14 days before the start of the course or earlier if the maximum number of students is reached.

Remarks
A minimum of 5 participants is required.