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Prospectus

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Capillary Electrophoresis-Mass Spectrometry for Metabolomics

Course
2021-2022

Admission requirements

Master students BPS. The course is also open to third-year BPS students and to other MSc students with a basic background in analytical chemistry or separation sciences.

Description

The main objective of this course is to provide you with an overview of the main capillary electrophoresis-mass spectrometry (CE-MS) approaches used for metabolomics, including their working/separation mechanisms.
The course will be composed of both lectures and a grant writing assignment. Lectures are focused on capillary electrophoresis separation modes, electrospray ionization mass spectrometry, interfacing techniques for coupling CE to MS, comparison of this approach with other analytical techniques and (volume-restricted) metabolomics applications.
Apart from (following) lectures, you will work individually on writing a research grant proposal using the criteria as set out for constructing a Veni research proposal for the Dutch Organization for Scientific Research (NWO). The scientific approach should be at least contain capillary electrophoresis as one of the approaches. You are totally free to come up with a challenging and innovative biological/clinical question or application. For the effective design of such a research proposal, a proper background in the basics of CE-MS is required.

This course aims to:

  • give an introduction into the basics of capillary electrophoresis-mass spectrometry (CE-MS)

  • explain the concept of metabolomics and how to set-up a CE-MS-based analytical workflow to address a biological question

  • discuss the strengths and limitations of CE-MS for metabolomics studies

  • learn the student how to design a Veni grant proposal, in which CE(-MS) plays a key role, according to NWO guidelines

Course objectives

At the end of this course the student is able to:

  • give an introduction into the basics of capillary electrophoresis-mass spectrometry (CE-MS)

  • explain the concept of metabolomics and how to set-up a CE-MS-based analytical workflow to address a biological question

  • discuss the strengths and limitations of CE-MS for metabolomics studies

  • to design a Veni grant proposal, in which CE(-MS) plays a key role, according to NWO guidelines

Timetable

This course is scheduled for semester 1, period 2.
A detailed course schedule will be published on Brightspace and a specific MS Teams will be made for this course.

Mode of instruction

Lectures, tutorials and self-tuition.

Assessment method

The assessment is based on the scientific quality of your research proposal (70% of final grade) and of your presentation of the research proposal (30% of final grade).
For each component, the grade should be ≥ 5.0, and the final/average grade should be ≥ 5.5 to successfully complete this course.

Reading list

The following book is recommended for this course: Capillary Electrophoresis-Mass Spectrometry for Metabolomics by Rawi Ramautar, ISBN: 9781788012737, publisher: The Royal Society of Chemistry (http://pubs.rsc.org/bookshop/search?searchtext=ramautar)

Registration

Application via uSis for both the course and exam is mandatory. Registration for the course closes 14 days before the start of the course or earlier when the maximum number of students is reached. Registration for the exam closes 7 days before the exam date or earlier when the maximum number of students is reached.

Contact

Coordinator: Dr. Rawi Ramautar (r.ramautar@lacdr.leidenuniv.nl), Associate Professor and PI of Biomedical Microscale Analytics (https://www.universiteitleiden.nl/en/science/drug-research/systems-pharmacology/biomedical-microscale-analytics).

Remarks

A minimum of 5 and a maximum of 24 participants applies to this course. Placement is based on the registration date.
The lectures will be given in hybrid format, i.e. online and on-campus.

This information is without prejudice. Alterations can be made for next year.