Prospectus

nl en

Translational Neurogenetics

Course
2019-2020

Admission requirements

  • Successful completion of 3120321PPY (How To Write A Research Proposal) is strongly recommended.

  • Student is assumed to be familiar with knowledge described in: Human Molecular Genetics (Strachan & Read): Chapters 4, 13, 14, 15, 16, 20, 21.

Description

Period: 6 January 2020 - 31 January 2020

This four-week course will take you through the successive steps in the identification and characterization of the gene defect(s) in various monogenic and multifactorial diseases, with a focus on neurogenetic diseases.

Neurogenetic diseases form a major medical, personal and societal burden in the population. Many of the diseases show an early onset, while we see a steep increase in various neurodegenerative disorders in the ageing population. In Leiden, there are several strong clinical-molecular research groups working on episodic (e.g. migraine, stress/depression), neurodegenerative (e.g. Huntington, Parkinson, Alzheimer) and neuromuscular (e.g. Duchenne, FSHD) diseases. These collaborative networks provide unique opportunities for translational research “from bench to bedside – and back”. In this course, much emphasis is given to the functional consequences of the mutations in the DNA (i.e. genotype) – on the RNA, protein and metabolite level (i.e. phenotype). Thus, subsequent to gene and mutation identification, their functional consequences need to be studied in various in vitro and in vivo model systems. Our main emphasis will be on transgenic and (conditional) knock-out mouse models. These models are studied with cutting edge molecular, (electro)physiological, behavioural and imaging techniques. How knowledge of genetic and functional studies can lead to the identification of targets for the development of therapeutical strategies will be discussed.

The flow of the course is as follows:
In the* first part of the course*, a thorough theoretical overview of the various steps in gene discovery and functional characterization is given. Important issues such as collection and diagnosis of families and populations with a (neuro)genetic disease, modern (genome-wide) gene identification strategies (Next Generation Sequencing), genome editing (CRISPR/Cas), functional assays, bioinformatics, model systems and therapeutic strategies will be discussed in lectures, demonstrations and self-study assignments.
In the second part of the course, students participate in ongoing disease-oriented projects to get a better insight in modern translational neurogenetics research. Specific emphasis is given to the complex neurobiological processes at the basis of the observed clinical phenotype in patients and mouse models. The experience gained will be used to design research proposals to be presented at the end of the week in a mini-symposium.

This course will particularly work on:

Research competences:
Defining a research question, writing a research proposal, choosing appropriate techniques

Professional competences:
Collaborating with peers, commitment, motivation and drive, digesting other people’s opinions

Course objectives

The student:

  • has an overall understanding of the theoretical and practical features of family studies in (genetic) diseases

  • has an overview and knowledge of strategies to identify genes

  • has an overview and knowledge of functional assays and models for the development of treatment and prevention strategies of genetic diseases

  • can convey this knowledge and views to the other students

  • can implement this knowledge in the design of a written research proposal and an oral presentation about a chosen subject.

Timetable

All course and group schedules are published on our LUMC scheduling website or on the LUMC scheduling app.

Mode of Instruction

  • Lectures

  • Working classes

  • Self-study assignments

Course load

Total course load is the amount of EC’s multiplied with 28 hours.

Assessment method

  • Student behaviour (motivation, independency, oral reporting, participation in discussion)

  • The research proposal

  • The student’s oral presentation of his assignment during a plenary session with all course participants

Registration

Registration for FOS courses, H2W, Scientific Conduct, How to start, Course on Animal Science , and CRiP and Adv concepts courses takes place in lottery rounds in the beginning of July. After the lottery rounds: if you want to register for a course you are kindly asked to contact the student administration at masterbw-courses@lumc.nl.

Contact

masterbms-courses@lumc.nl

Remarks

This course will be given in parallel with the course: ‘Translational Neurogenetics’. A substantial number of lectures will be given for the combined group.